Soluble humic acid suppresses plant immunity and ethylene to promote soybean nodulation

Abstract Symbiotic nitrogen fixation (SNF) is a crucial process for nitrogen geochemical cycling and plant‐microbe interactions. Water‐soluble humic acid (WSHM), an active component of soil humus, has been shown to promote SNF in the legume‐rhizobial symbiosis, but its molecular mechanism remains largely unknown. To reveal the SNF‐promoting mechanism, we conducted transcriptomic analysis on soybean treated with WSHM. Our findings revealed that up‐ and downregulated differentially expressed genes (DEGs) were mainly involved in plant cell‐wall/membrane formation and plant defence/immunity in the early stage, while the late stage was marked by the flavonoid synthesis and ethylene biosynthetic process. Further study on representative DEGs showed that WSHM could inhibit GmBAK1d‐mediated immunity and BR signalling, thereby promoting rhizobial colonisation, infection, and nodulation, while not favoring pathogenic bacteria colonisation on the host plant. Additionally, we also found that the ethylene pathway is necessary for promoting the soybean nodulation by WSHM. This study not only provides a significant advance in our understanding of the molecular mechanism of WSHM in promoting SNF, but also provides evidence of the beneficial interactions among the biostimulator, host plant, and soil microbes, which have not been previously reported.