神经干细胞
转染
纳米技术
核酸
免疫原性
生物
计算生物学
细胞生物学
干细胞
计算机科学
材料科学
基因
生物化学
免疫学
免疫系统
作者
Yu-Wen Chao,Yen-Lurk Lee,Ching-San Tseng,L Wang,Kuo‐Chiang Hsia,Huatao Chen,Jean‐Michel Fustin,Sayma Azeem,Tzu-Tung Chang,Chiung-Ya Chen,Fan-Che Kung,Yi‐Ping Hsueh,Yi‐Shuian Huang,Hsu‐Wen Chao
出处
期刊:ACS Nano
[American Chemical Society]
日期:2024-01-29
卷期号:18 (6): 4822-4839
被引量:2
标识
DOI:10.1021/acsnano.3c09608
摘要
Efficiently delivering exogenous materials into primary neurons and neural stem cells (NSCs) has long been a challenge in neurobiology. Existing methods have struggled with complex protocols, unreliable reproducibility, high immunogenicity, and cytotoxicity, causing a huge conundrum and hindering in-depth analyses. Here, we establish a cutting-edge method for transfecting primary neurons and NSCs, named teleofection, by a two-step process to enhance the formation of biocompatible calcium phosphate (CaP) nanoparticles. Teleofection enables both nucleic acid and protein transfection into primary neurons and NSCs, eliminating the need for specialized skills and equipment. It can easily fine-tune transfection efficiency by adjusting the incubation time and nanoparticle quantity, catering to various experimental requirements. Teleofection's versatility allows for the delivery of different cargos into the same cell culture, whether simultaneously or sequentially. This flexibility proves invaluable for long-term studies, enabling the monitoring of neural development and synapse plasticity. Moreover, teleofection ensures the consistent and robust expression of delivered genes, facilitating molecular and biochemical investigations. Teleofection represents a significant advancement in neurobiology, which has promise to transcend the limitations of current gene delivery methods. It offers a user-friendly, cost-effective, and reproducible approach for researchers, potentially revolutionizing our understanding of brain function and development.
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