适体
芬太尼
化学
分析物
指数富集配体系统进化
生物流体
生物传感器
免疫分析
药理学
纳米技术
色谱法
抗体
核糖核酸
生物化学
医学
分子生物学
基因
生物
材料科学
免疫学
作者
Juan Canoura,Yingzhu Liu,Obtin Alkhamis,Yi Xiao
标识
DOI:10.1021/acs.analchem.3c04104
摘要
Fentanyl is a widely abused analgesic and anesthetic drug with a narrow therapeutic window that creates easy opportunities for overdose and death. Rapid, accurate, and sensitive fentanyl detection in biosamples is crucial for therapeutic drug monitoring and overdose diagnosis. Unfortunately, current methods are limited to either sophisticated laboratory-based tests or antibody-based immunoassays, which are prone to false results and are mainly used with urine samples. Here, we have utilized library-immobilized SELEX to isolate new aptamers─nucleic acid–based bioreceptors that are well-suited for biosensing─that can specifically bind fentanyl under physiological conditions. We isolated multiple aptamers with nanomolar affinity and excellent specificity against dozens of interferents and incorporated one of these into an electrochemical aptamer-based sensor that can rapidly detect fentanyl at clinically relevant concentrations in 50% diluted serum, urine, and saliva. Given the excellent performance of these sensors, we believe that they could serve as the basis for point-of-care devices for monitoring fentanyl during medical procedures and determining fentanyl overdose.
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