唾液酸
唾液酸糖蛋白
胎球蛋白
化学
硼酸
唾液糖蛋白
离解(化学)
唾液酸酶
生物分子
N-乙酰神经氨酸
生物化学
色谱法
组合化学
糖蛋白
有机化学
神经氨酸酶
酶
作者
Yuji Horiguchi,Masato Yasuura,Hiroki Ashiba,Zheng Lin Tan,Takashi Fukuda
出处
期刊:Sensors
[MDPI AG]
日期:2024-02-07
卷期号:24 (4): 1080-1080
摘要
An overexpression of sialic acid is an indicator of metastatic cancer, and selective detection of sialic acid shows potential for cancer diagnosis. Boronic acid is a promising candidate for this purpose because of its ability to specifically bind to sialic acid under acidic conditions. Notably, the binding strength can be easily modulated by adjusting the pH, which allows for a simple dissociation of the bound sialic acid. In this study, we developed 5-boronopicolinic acid (5-BPA)-modified magnetic particles (BMPs) to selectively capture sialic acid biomolecules. We successfully captured fetuin, a well-known sialoglycoprotein, on BMPs at >104 molecules/particle using an acetate buffer (pH 5.0). Facile dissociation then occurred when the system was changed to a pH 7.6 phosphate buffer. This capture-and-release process could be repeated at least five times. Moreover, this system could enrich fetuin by more than 20 times. In summary, BMPs are functional particles for facile purification and concentration through the selective capture of sialic acid proteins and can improve detection sensitivity compared with conventional methods. This technology shows potential for the detection of sialic acid overexpression by biological particles.
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