Spectrophotometric Determination of Acetamiprid Using Gold Nanoparticles (AuNPs) with Optimized Truncated Aptamers

Acetamiprid is mainly used to control pests. However, the residues of acetamiprid have resulted in significant negative effects on mammals. Many researchers are working on developing acetamiprid detection methods. In our study, four truncated aptamers were designed based on the secondary structure of the original aptamer. Molecular docking simulation of truncated aptamers confirmed that all of the truncated aptamers bind to acetamiprid, and their binding sites were identified. The affinity, sensitivity, and specificity of these truncated aptamers were measured by a spectrophotometric method with gold nanoparticles (AuNPs). Acetamiprid aptamers with 17-mer (Apt-J2) and 16-mer (Apt-J4) were successfully obtained, which show higher detection sensitivity and satisfactory specificity. Additionally, a spectrophotometric detection route superior to the original aptamer was explored, and the linear ranges were from 1 to 70 μM and 0.5 to 40 μM, respectively. The truncated aptamers were applied to the analysis of lake water, indicating that the aptamers have potential for practical analysis.