Identification and Quantification of Microexons Using Bulk and Single-Cell RNA-Seq Data

The analysis of RNA-seq has greatly improved the characterization and understanding of the transcriptome. In particular, RNA-seq experiments have extended catalogs of alternative splicing events. However, the analysis of RNAs-seq data for detection and quantification of microexons, extremely short exons of length up to 30 nt, require specialized computational workflows. Here, we describe MicroExonator, a reproducible computational workflow for microexon splicing analysis using bulk or single-cell RNA-seq data.