Co-digestion of sulfur-rich vegetable waste with waste activated sludge enhanced phosphorus release and hydrogenotrophic methanogenesis

Anaerobic co-digestion of sulfur-rich vegetable waste (SVW) with waste activated sludge (WAS) and the underlying mechanisms associated with methane production and phosphorus (P) release were investigated. Four types of SVW (Chinese cabbage, cabbage, rapeseed cake, and garlic) were utilized for co-digestion with WAS, and the methane yield increased by 7.3%-35.3%; in the meantime, the P release amount from WAS was enhanced by 9.8%-24.9%. The organic carbon in SVW promoted methane production, while organic sulfur and the formation of FeS facilitated P release. Among the four types of SVW, rapeseed cake was identified as the most suitable co-digestion substrate for enhancing both methane production and P release due to its balanced nutrients and relatively high sulfur content. Syntrophic bacteria working with hydrogenotrophic methanogens, iron-reducing bacteria, sulfate-reducing bacteria, and hydrogenotrophic methanogens were enriched. Metabolic pathways related to sulfate reduction and methanogenesis were facilitated, especially hydrogenotrophic methanogenesis. Enzymes involved in hydrogenotrophic methanogenesis were promoted by 76.05%-407.98% with the addition of Chinese cabbage, cabbage, or rapeseed cake. This study provides an eco-friendly technology for promoting P resource and energy recovery from WAS and an in-depth understanding of the corresponding microbial mechanisms.