微泡
多囊卵巢
内分泌学
小RNA
内科学
外体
生物
卵巢
抗苗勒氏激素
无排卵
发情周期
激素
医学
胰岛素
胰岛素抵抗
遗传学
基因
作者
Jianzhong Xiao,Zhirong Zhang,Min Hou,Xiaohe Yang,Linlin Cui
标识
DOI:10.1096/fj.202201940rr
摘要
Abstract Anovulation is the main feature of infertile women with polycystic ovary syndrome (PCOS), and there is very limited understanding of the role of plasma exosomes and miRNAs in it. To identify the effect of PCOS patients' plasma exosomes and exosomal miRNAs, we isolated plasma exosomes of PCOS patients and normal women and injected into 8‐week‐old ICR female mice via tail vein. The changes in estrus cycle, serum hormone levels, and ovarian morphology were observed. KGN cells were cultured and transfected with mimics and inhibitors of differentially expressed exosomal miRNAs (miR‐18a‐3p, miR‐20b‐5p, miR‐106a‐5p, miR‐126‐3p, and miR‐146a‐5p) and then tested for steroid hormone synthesis, proliferation, and apoptosis. The results showed that female ICR mice injected with plasma exosomes from PCOS patients presented ovarian oligo‐cyclicity. Hormone synthesis and proliferation of granulosa cells were affected by differentially expressed PCOS plasma‐derived exosomal miRNAs, of which miR‐126‐3p having the most evident effect. MiR‐126‐3p affected the proliferation of granulosa cells by inhibiting PDGFRβ and its downstream PI3K‐AKT pathway. Our results demonstrated plasma exosomes and contained miRNAs in PCOS patients affect the estrus cycle of mice, hormone secretion, and proliferation of granulosa cells. This study provides a novel understanding about the function of plasma exosomes and exosomal miRNAs in PCOS.
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