成熟
采后
基因沉默
番茄红素
调节器
乙烯
园艺
基因
基因表达
生物
植物
细胞生物学
类胡萝卜素
生物化学
催化作用
作者
Zhiqiang Wang,Qing Wang,Wenhui Duan,Lanhuan Meng,Jiangkuo Li,Hongmiao Song,Xiangbin Xu
标识
DOI:10.1016/j.postharvbio.2022.112132
摘要
Fruit ripening of tomato is regulated by NAC (NAM, ATAF1/2, and CUC2) domain proteins such as NOR-like1, SlNAC1, and SlNAC4. Acts as an up-stream regulator of NAC genes, the high abundance of miR164 might eliminate the impact of NAC on the ripening of fleshy fruit. In the present study, the STTM-miR164a (silencing miR164a by short tandem target mimic) and OE-miR164a (overexpression of miR164a) tomato lines were generated. Compared with those of silenced miR164a, overexpression of miR164a decreased ethylene production and lycopene accumulation, and delayed pre-and postharvest ripening of fruit. The expression analysis of target genes of miR164a revealed that the NAM3 (Solyc06g069710), GOB (Solyc07g062840) and NAC1 (Solyc07g066330) were involved in regulating fruit ripening.
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