Valorization of Hakka Huangjiu lees: Optimization of ultrasound-assisted enzymatic extraction and identification of angiotensin I-converting enzyme inhibitory peptides

酒糟 萃取(化学) 抑制性突触后电位 化学 生物化学 色谱法 肾素-血管紧张素系统 鉴定(生物学) 生物 内分泌学 植物 发酵 血压
作者
Shuchun Yang,Hong Wang,Weidong Bai,Wenhong Zhao,Gongliang Liu,Xiaoyan Liu
出处
期刊:Food and Bioproducts Processing [Elsevier BV]
卷期号:146: 77-88
标识
DOI:10.1016/j.fbp.2024.05.010
摘要

Angiotensin I-converting enzyme (ACE) inhibitory peptides of natural origin have attracted wide attention due to their easy metabolism and low toxicity. The aim of this study is to effectively utilize Hakka Huangjiu lees (HHL) to prepare peptides with high ACE inhibitory activity. In this study, ultrasound-assisted enzymatic extraction of peptides from HHL was optimized using single-factor and response surface experiments. Subsequently, the HHL hydrolysate was further isolated and purified based on the ACE inhibitory activity by ultrafiltration and chromatography methods. Finally, the molecular docking was used to explore the relationship between peptide structure and function. The results showed that the optimal extraction conditions were ultrasonic time 10 min, ultrasonic power 200 W, alcalase/substrate ratio (E/S) 6000 U/g, and enzymatic hydrolysis time 4 h. Under the optimal conditions, the extraction rate of peptides from dried and defatted HHL was 31.25%, which was comparable to the 7.10% of the raw materials. Subsequently, three novel ACE inhibitory peptides were prepared from HHL hydrolysate and were identified as Ser-Asn-Tyr-Phe-Pro-Arg-Pro-Trp (SNYFPRPW), Trp-Leu-Gly-Tyr-Pro-Arg (WLGYPR), and Asp-Leu-Arg-Tyr-Trp (DLRYW), with the IC50 values of 45.36, 31.16 and 24.32 Μm, respectively. In addition, the three peptides all had high ACE activity, which was due to their effective interaction with the ACE active site through hydrogen bonding and hydrophobic interactions. Therefore, the ACE inhibitory peptides extracted from HHL, especially SNYFPRPW, WLGYPR and DLRYW, can be valuable candidates for the production of antihypertensive nutraceuticals and functional foods, providing the basis for alternative valorized product streams in the fermentation industry.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
3秒前
4秒前
4秒前
hhh发布了新的文献求助10
5秒前
6秒前
凩飒给凩飒的求助进行了留言
7秒前
一程发布了新的文献求助10
7秒前
巴黎的防发布了新的文献求助10
8秒前
义气沉鱼发布了新的文献求助10
10秒前
Komorebi完成签到 ,获得积分10
11秒前
11秒前
搜集达人应助小红的忧伤采纳,获得10
12秒前
daying完成签到,获得积分10
14秒前
半糖糖完成签到,获得积分10
14秒前
15秒前
竹简发布了新的文献求助10
15秒前
16秒前
愤怒的小马完成签到,获得积分10
18秒前
半糖糖发布了新的文献求助10
19秒前
21秒前
Amanda发布了新的文献求助10
21秒前
秦文完成签到 ,获得积分10
25秒前
王WW发布了新的文献求助10
25秒前
淡定幻翠发布了新的文献求助10
28秒前
28秒前
29秒前
星辰大海应助白小超人采纳,获得10
30秒前
星辰大海应助Amanda采纳,获得10
30秒前
32秒前
32秒前
36秒前
桃之夭夭发布了新的文献求助10
38秒前
43秒前
43秒前
44秒前
44秒前
在水一方应助怕黑凤采纳,获得10
45秒前
46秒前
吴小台呀发布了新的文献求助10
47秒前
高分求助中
Ophthalmic Equipment Market by Devices(surgical: vitreorentinal,IOLs,OVDs,contact lens,RGP lens,backflush,diagnostic&monitoring:OCT,actorefractor,keratometer,tonometer,ophthalmoscpe,OVD), End User,Buying Criteria-Global Forecast to2029 2000
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 588
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3962475
求助须知:如何正确求助?哪些是违规求助? 3508497
关于积分的说明 11141410
捐赠科研通 3241254
什么是DOI,文献DOI怎么找? 1791445
邀请新用户注册赠送积分活动 872863
科研通“疑难数据库(出版商)”最低求助积分说明 803417