大肠杆菌
生物合成
烟酰胺
核糖苷
NAD+激酶
烟酰胺腺嘌呤二核苷酸
化学
辅因子
腈水合酶
生物化学
催化作用
酶
基因
作者
Zhongshi Huang,Xinglong Wang,Ning Li,Fuqiang Song,Jingwen Zhou
标识
DOI:10.1016/j.biortech.2023.128953
摘要
Nicotinamide riboside (NR), a key biosynthetic precursor of NAD+, is receiving increasing attention because of its role. In this study, a whole-cell catalysis method to efficiently synthesize NR was established. First, the performance of 5'-nucleotidase (UshA) from Escherichia coli was confirmed to have high catalytic activity to synthesize NR. Then, the endogenous NR degradation pathway was detected, and the genes (rihA, rihB, and rihC) involved in NR degradation were knocked out, which enabled NR biosynthesis. In addition, the important role of the signal peptide of UshA in NR transport had been confirmed. Subsequently, nitrile hydratase was introduced to achieve the conversion of 3-cyanopyridine to NR. Finally, the NR titer reached 25.6 and 29.8 g/L with nicotinamide and 3-cyanopyridine, respectively, as substrates in a 5-L bioreactor, the efficient biosynthesis of NR in E. coli by using nicotinamide and 3-cyanopyridine.
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