向日葵
向日葵
转录组
生物
基因
亚油酸
脂肪酸去饱和酶
油酸
脂肪酸
食品科学
基因表达
葵花籽油
生物化学
植物
园艺
多不饱和脂肪酸
作者
Qixiu Huang,Lijun Xiang,Li Zhang,Yushanjiang Maimaiti,Wenfang Luo,Zhonghua Lei
出处
期刊:Agronomy
[MDPI AG]
日期:2023-03-16
卷期号:13 (3): 885-885
标识
DOI:10.3390/agronomy13030885
摘要
Sunflower (Helianthus annuus L.) is an important oil crop with rich nutrients, and genetically engineered breeding has become an important way to improve its quality. In this study, five varieties of oilseed sunflower were analyzed for fatty acid (FA) content. The seed embryos of one of the high oleic acid (OA) varieties were transcriptome sequenced at different stages. The results showed that OA synthesis dominated the unsaturated fatty acid (UFA) synthesis pathways in seed embryos. Substantially differentially expressed genes were detected at various post-flowering stages. Specifically, the up-regulated gene numbers were highest at 10 d after flowering, while most genes were down-regulated at 20 d after flowering. The enriched genes were rather consistent with almost all experimental groups exhibiting enrichment to the FAD2 gene. The expression of FAD2 was highly negatively correlated with the expressions of FAD6, FAD3, and FAD7. During seed embryo development, the expression level of FAD2 was highly negatively correlated with the final OA content and was highly positively correlated with the final linoleic acid (LA) content. This suggests that the FAD2 is a key enzyme catalyzing the OA to LA conversion.
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