生物
插层(化学)
细胞生物学
延伸率
上皮
管腔(解剖学)
细胞凋亡
细胞
机制(生物学)
单层
生物物理学
生物化学
材料科学
化学
遗传学
认识论
极限抗拉强度
哲学
无机化学
冶金
作者
Alexander Pfannenstein,Ian G. Macara
标识
DOI:10.1016/j.devcel.2023.04.009
摘要
Summary
The luminal epithelium of the mammary gland is organized into monolayers; however, it originates from multilayered terminal end buds (TEBs) during development. Although apoptosis provides a plausible mechanism for cavitation of the ductal lumen, it doesn't account for ductal elongation behind TEBs. Spatial calculations in mice suggest that most TEB cells integrate into the outermost luminal layer to generate elongation. We developed a quantitative cell culture assay that models intercalation into epithelial monolayers. We found that tight junction proteins play a key role in this process. ZO-1 puncta form at the new cellular interface and resolve into a new boundary as intercalation proceeds. Deleting ZO-1 suppresses intercalation both in culture and in cells transplanted into mammary glands via intraductal injection. Cytoskeletal rearrangements at the interface are critical for intercalation. These data identify luminal cell rearrangements necessary for mammary development and suggest a mechanism for integration of cells into an existing monolayer.
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