High concentrations of Cu2+ exceeding what the required level for the cellular process can changes the properties of protein, affects the normal function of the body, but also affect the body's metabolism, so that the body's immunity appears to decline. Detection of Cu2+ is important for the environmental monitoring. Here, a fluorescence probe that uses heavy metal-free and properties-superior CuInS2/ZnS was proposed for detection of Cu2+. The fluorescence intensity of CuInS2/ZnS quantum dots (QDs) decreased obviously after Cu2+ were added, and the intensity with the concentration of Cu2+ showed excellent linear relation within the scope of 0–50 μM, the LOD down to 0.45 μM, a possible detection mechanism was analyzed. The QDs was further applied to the detection of Cu2+ in drinking water, manifesting the foreground of the current strategy.