Spatial proximity of tumor-immune interactions predicts patient outcome in hepatocellular carcinoma

肝细胞癌 免疫系统 肿瘤微环境 CD8型 肝癌 癌症 医学 癌症研究 转录组 肿瘤科 生物 内科学 基因 免疫学 基因表达 遗传学
作者
Evan Maestri,Noémi Kedei,Subreen A. Khatib,Marshonna Forgues,Kris Ylaya,Stephen M. Hewitt,Limin Wang,Jittiporn Chaisaingmongkol,Mathuros Ruchirawat,Lichun Ma,Xin Wei Wang
出处
期刊:Hepatology [Lippincott Williams & Wilkins]
卷期号:79 (4): 768-779 被引量:11
标识
DOI:10.1097/hep.0000000000000600
摘要

Background and Aims: The fitness and viability of a tumor ecosystem are influenced by the spatial organization of its cells. We aimed to study the structure, architecture, and cell-cell dynamics of the heterogeneous liver cancer tumor microenvironment using spatially resolved multiplexed imaging. Approach and Results: We performed co-detection by indexing multiplexed immunofluorescence imaging on 68 HCC biopsies from Thai patients [(Thailand Initiative in Genomics and Expression Research for Liver Cancer (TIGER-LC)] as a discovery cohort, and then validated the results in an additional 190 HCC biopsies from Chinese patients [Liver Cancer Institute (LCI)]. We segmented and annotated 117,270 and 465,632 cells from the TIGER-LC and LCI cohorts, respectively. We observed 4 patient groups of TIGER-LC (IC1, IC2, IC3, and IC4) with distinct tumor-immune cellular interaction patterns. In addition, patients from IC2 and IC4 had much better overall survival than those from IC1 and IC3. Noticeably, tumor and CD8 + T-cell interactions were strongly enriched in IC2, the group with the best patient outcomes. The close proximity between the tumor and CD8 + T cells was a strong predictor of patient outcome in both the TIGER-LC and the LCI cohorts. Bulk transcriptomic data from 51 of the 68 HCC cases were used to determine tumor-specific gene expression features of our classified subtypes. Moreover, we observed that the presence of immune spatial neighborhoods in HCC as a measure of overall immune infiltration is linked to better patient prognosis. Conclusions: Highly multiplexed imaging analysis of liver cancer reveals tumor-immune cellular heterogeneity within spatial contexts, such as tumor and CD8 + T-cell interactions, which may predict patient survival.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
2秒前
SYLH应助张成采纳,获得10
2秒前
2秒前
优雅夜雪发布了新的文献求助10
3秒前
4秒前
4秒前
宋坤应助清新的访冬采纳,获得10
4秒前
琅琊为刃发布了新的文献求助10
5秒前
X先生完成签到,获得积分10
5秒前
5秒前
Vizz发布了新的文献求助10
5秒前
难过冷玉发布了新的文献求助20
5秒前
9秒前
kk发布了新的文献求助10
9秒前
10秒前
深情安青应助Dann采纳,获得10
13秒前
毒盐完成签到,获得积分20
14秒前
15秒前
zz完成签到,获得积分10
16秒前
16秒前
16秒前
体贴的乐荷完成签到,获得积分10
17秒前
小郑小郑完成签到 ,获得积分10
17秒前
zu发布了新的文献求助10
17秒前
jjb完成签到,获得积分10
17秒前
十一发布了新的文献求助20
19秒前
19秒前
JamesPei应助独特冰安采纳,获得10
19秒前
yy完成签到,获得积分20
19秒前
Horizon完成签到,获得积分10
19秒前
zhangzhang发布了新的文献求助10
21秒前
slin_sjtu发布了新的文献求助10
21秒前
思源应助。。。采纳,获得10
22秒前
研友_VZG7GZ应助花花采纳,获得10
23秒前
盛乾衣发布了新的文献求助10
25秒前
Hexagram发布了新的文献求助10
25秒前
科研通AI2S应助阿俊1212采纳,获得10
26秒前
yqqqqq发布了新的文献求助10
26秒前
26秒前
高分求助中
Picture Books with Same-sex Parented Families: Unintentional Censorship 1000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 310
The Moiseyev Dance Company Tours America: "Wholesome" Comfort during a Cold War 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3980154
求助须知:如何正确求助?哪些是违规求助? 3524160
关于积分的说明 11220159
捐赠科研通 3261641
什么是DOI,文献DOI怎么找? 1800734
邀请新用户注册赠送积分活动 879263
科研通“疑难数据库(出版商)”最低求助积分说明 807232