Liamocin biosynthesis is induced by an autogenous host acid activation in Aureobasidium melanogenum

柠檬酸 互补 生物合成 生物化学 ATP柠檬酸裂解酶 基因 基因表达 转录因子 生物 化学 柠檬酸合酶 突变体
作者
Mei Zhang,Xin Wei,Peng Wang,Zhe Chi,Guang‐Lei Liu,Zhen‐Ming Chi
出处
期刊:Biotechnology Journal [Wiley]
卷期号:19 (1)
标识
DOI:10.1002/biot.202200440
摘要

Abstract It has been known that maximal liamocin production must be carried out at low environmental pH (around 3.0). In this study, it was found that the low pH was mainly caused by the secreted citric acid which is one precursor of acetyl‐CoA for liamocin biosynthesis. Determination of citric acid in the culture, deletion, complementation and overexpression of the CEXA gene encoding specific citrate exporter demonstrated that the low pH was indeed caused by the secreted citric acid. Deletion, complementation and overexpression of the ACL gene encoding ATP‐citric acid lyase and effects of different initial pHs and added citric acid showed that the low pH in the presence of citric acid was suitable for lysis of intracellular citric acid, liamocin production and expression of the PACC gene encoding the pH signaling transcription factor PacC. This meant that the PACC gene was an acid‐expression gene. Deletion, complementation and overexpression of the PACC gene indicated that expression of the key gene cluster GAL1‐EST1‐PKS1 for liamocin biosynthesis was driven by the pH signaling transcription factor PacC and there was weak nitrogen catabolite repression on liamocin biosynthesis at the low pH. That was why liamocin biosynthesis was induced at a low pH in the presence of citric acid. The mechanisms of the enhanced liamocin biosynthesis by the autogenous host acid activation, together with the pH signaling pathway, were proposed.
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