细胞凋亡
体内
蛋白激酶B
体外
PI3K/AKT/mTOR通路
癌症研究
化学
膀胱癌
细胞生物学
癌症
生物
医学
内科学
生物化学
遗传学
作者
Mingfang Weng,Zhen Deng,Shuijing Huang,Xiaowen Lin,Na Xu,Xinhui Sun,Weizhen Wu,Jun Lü,Dong Wang
摘要
Abstract Fraxetin, a natural compound extracted from the Chinese herb Cortex Fraxini , is reported to boast extensive antitumor properties in various cancers. However, whether fraxetin exhibited an anticancer effect on bladder cancer remains unknown. In this study, cell counting kit‐8 was utilized to detect cell viability. Flow cytometry analysis was performed for cell apoptosis analysis. Western blot analysis and real‐time PCR were used to ascertain gene expression analysis. A mouse bladder cancer xenograft model was established and subjected to fraxetin treatment. Fraxetin reduced the viability of bladder cancer cells, induced apoptosis in vitro, and inhibited the growth of bladder cancer in vivo. Fraxetin inhibited the Akt pathway in J82 cells. In conclusion, the growth inhibitory properties of fraxetin against bladder cancer may be mediated via an Akt inhibitory effect and cell apoptosis promotion.
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