Analysis of sputum microbial flora in chronic obstructive pulmonary disease patients with different phenotypes during acute exacerbations

慢性支气管炎 慢性阻塞性肺病 医学 支气管扩张 表型 肺炎链球菌 内科学 免疫学 微生物学 生物 抗生素 病理 肺结核 基因 遗传学
作者
Xiaoyan Mao,Yao Li,Pengfei Shi,Ziwei Zhu,Juan Sun,Yu Xue,Zongren Wan,Dan Yang,Ting Ma,Jipeng Wang,Rong Zhu
出处
期刊:Microbial Pathogenesis [Elsevier]
卷期号:184: 106335-106335 被引量:8
标识
DOI:10.1016/j.micpath.2023.106335
摘要

Increasing studies have shown that the imbalance of the respiratory microbial flora is related to the occurrence of COPD, the severity and frequency of exacerbations and mortality.However, it remains unclear how the sputum microbial flora differs during exacerbations in COPD patients manifesting emphysema phenotype, chronic bronchitis with emphysema phenotype and asthma-COPD overlap phenotype.Sputum samples were obtained from 29 COPD patients experiencing acute exacerbations who had not received antibiotics or systemic corticosteroids within the past four weeks.Patients were divided into three groups;emphysema phenotype(E);chronic bronchitis with emphysema phenotype(B+E) and asthma-COPD overlap phenotype(ACO).We utilized metagenomic Next Generation Sequencing (mNGS) technology to analyze the sputum microbial flora in COPD patients with different phenotypes during exacerbations.There was no significant difference in alpha diversity and beta diversity among three groups.The microbial flora composition was similar in all three groups during exacerbations except for a significant increase in Streptococcus mitis in ACO.Through network analysis,we found Candidatus Saccharibacteria oral taxon TM7x and Fusobacterium necrophorum were the core nodes of the co-occurrence network in ACO and E respectively.They were positively correlated with some species and play a synergistic role.In B+E,Haemophilus pittmaniae and Klebsiella pneumoniae had a synergistic effect.Besides,some species among the three groups play a synergistic or antagonistic role.Through Spearman analysis,we found the relative abundance of Streptococcus mitis was negatively correlated with the number of hospitalizations in the past year(r = -0.410,P = 0.027).We also observed that the relative abundance of Prevotella and Prevotella melaninogenica was negatively correlated with age(r = -0.534,P = 0.003;r = -0.567,P = 0.001),while the relative abundance of Streptococcus oralis and Actinomyces odontolyticus was positively correlated with age(r = 0.570,P = 0.001;r = 0.480,P = 0.008).In addition,the relative abundance of Prevotella melaninogenica was negatively correlated with peripheral blood neutrophil ratio and neutrophil to lymphocyte ratio(r = -0.479,P = 0.009;r = -0.555,P = 0.002),while the relative abundance of Streptococcus sanguinis was positively correlated with peripheral blood neutrophil ratio and neutrophil to lymphocyte ratio (r = 0.450,P = 0.014;r = 0.501,P = 0.006).There was also a significant positive correlation between Oribacterium and blood eosinophil counts(r = 0.491,P = 0.007).Overall,we analyzed the sputum microbiota of COPD patients with different phenotypes and its relationship with clinical indicators, and explored the relationships between microbiota and inflammation in COPD.We hope to alter the prognosis of patients by inhibiting specific bacterial taxa related to inflammation and using guide individualized treatment in the future research.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
cometx完成签到,获得积分10
1秒前
研友_LOaovZ发布了新的文献求助30
2秒前
yyl完成签到,获得积分10
2秒前
科目三应助yuyu采纳,获得10
2秒前
AOtaku完成签到,获得积分10
2秒前
北方木棉发布了新的文献求助10
3秒前
cometx发布了新的文献求助10
3秒前
5秒前
cherry323完成签到,获得积分10
5秒前
5秒前
6秒前
hahhh7完成签到,获得积分10
6秒前
sun应助黑痴采纳,获得20
6秒前
yibiy完成签到,获得积分10
7秒前
8秒前
qiqi发布了新的文献求助10
9秒前
0h完成签到,获得积分10
9秒前
充电宝应助ljkshr采纳,获得10
9秒前
10秒前
www发布了新的文献求助10
11秒前
12秒前
12秒前
十九完成签到,获得积分10
12秒前
小蘑菇应助XXJ采纳,获得10
12秒前
桕火慢熬完成签到,获得积分10
12秒前
脑洞疼应助开始游戏55采纳,获得10
13秒前
陶陶发布了新的文献求助10
14秒前
xiaoxiaomi应助万木采纳,获得20
14秒前
鱼头关注了科研通微信公众号
14秒前
鱼头关注了科研通微信公众号
15秒前
15秒前
15秒前
言西早发布了新的文献求助20
15秒前
可爱的函函应助猴哥采纳,获得10
15秒前
Pan发布了新的文献求助10
16秒前
科目三应助科研通管家采纳,获得10
16秒前
16秒前
英俊的铭应助科研通管家采纳,获得10
16秒前
充电宝应助科研通管家采纳,获得10
16秒前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Conference Record, IAS Annual Meeting 1977 820
England and the Discovery of America, 1481-1620 600
Teaching language in context (Third edition) by Derewianka, Beverly; Jones, Pauline 550
電気学会論文誌D(産業応用部門誌), 141 巻, 11 号 510
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3583231
求助须知:如何正确求助?哪些是违规求助? 3152508
关于积分的说明 9492920
捐赠科研通 2854882
什么是DOI,文献DOI怎么找? 1569373
邀请新用户注册赠送积分活动 735182
科研通“疑难数据库(出版商)”最低求助积分说明 721082