Characterization of root endophytic Paenibacillus polymyxa isolates with biocontrol activity against Xanthomonas translucens and Fusarium graminearum

生物 多粘菌拟杆菌 镰刀菌 微生物学 拟杆菌 生物病虫害防治 几丁质酶 索拉尼镰刀菌 根腐病 铁载体 哈茨木霉 植物 细菌 16S核糖体RNA 基因 生物化学 遗传学
作者
Elaheh Taheri,Saeed Tarighi,Parissa Taheri
出处
期刊:Biological Control [Elsevier BV]
卷期号:174: 105031-105031 被引量:13
标识
DOI:10.1016/j.biocontrol.2022.105031
摘要

Fusarium crown rot (FCR), Fusarium head blight (FHB), and bacterial leaf streak (BLS) of wheat are important diseases worldwide. The lack of commercial resistant cultivars and harmful effects of using chemical poisons for controlling these diseases prompted scientists to use biological agents as eco-friendly strategies for disease management. Plants carry small community of microbes known as endophytes that have effect on plant growth regulation and also protect plants against phytopathogens. In this study, a total of 35 endophytic bacteria were isolated from wheat root. Two isolates were selected for further study based on their potential ability to control Xanthomonas translucens and Fusarium graminearum in vitro. Both isolates were identified as Paenibacillus polymyxa SeR8 and BR20 (F and B-type, respectively) according to the results of 16S rRNA gene sequencing, morphological, and physiological analysis. Light and scanning electron microscopy showed alternations in the F. graminearum structures in the presence of the endophytes. Production of protease, amylase, pectinase, cellulase, IAA, sensitivity to H2O2, biofilm formation, and phosphate solubilization in the type B was more than the type F. On the other hand, the production of lipase, catalase, ammonia, and siderophore by the F-type was more than the B-types, but they could not produce biosurfactants and ACC deaminase. None of them could produce chitinase. Kernel bioassay and mycotoxin analyses indicated that the F-type had better impact on protecting seeds and decreasing DON production. The percentages of FHB infection reduction, FCR severity reduction, and protection rate against BLS in the type F and B were 76.31, 64.10%; 67.66, 65.41%, and 54.96, 63.57%, respectively. Greenhouse experiments indicated that the biomass, 1000 grains weight, chlorophyll a, b, T, and carotenoids were increased in the plants treated with the endophytic bacteria. In our study, the B-type showed better antibacterial activity in comparison with the F-type, while the F type showed better antifungal activity than the B type. This is the first report that indicates the importance of types F and B of P. polymyxa in the biocontrol of Fusarium crown rot and bacterial leaf streak diseases.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
一一完成签到 ,获得积分10
刚刚
hp发布了新的文献求助10
2秒前
3秒前
姚世娇完成签到 ,获得积分10
5秒前
FashionBoy应助高手采纳,获得10
6秒前
meng完成签到,获得积分10
7秒前
chennn完成签到,获得积分10
7秒前
9秒前
10秒前
晗月完成签到,获得积分10
10秒前
情怀应助如意枫叶采纳,获得10
11秒前
量子星尘发布了新的文献求助10
13秒前
Akim应助SS采纳,获得10
14秒前
张雷应助清新的夜蕾采纳,获得20
14秒前
chennn发布了新的文献求助10
14秒前
罗一完成签到,获得积分10
16秒前
18秒前
丘比特应助wu采纳,获得10
21秒前
俏皮芷蕊发布了新的文献求助30
21秒前
称心的菲鹰完成签到,获得积分10
22秒前
碧蓝问安发布了新的文献求助10
23秒前
23秒前
打打应助ZZZ采纳,获得10
25秒前
29秒前
呆萌板凳发布了新的文献求助10
29秒前
hp关闭了hp文献求助
30秒前
31秒前
都选C完成签到,获得积分10
32秒前
壮观以松完成签到,获得积分10
32秒前
Liufgui应助郭小宝采纳,获得20
32秒前
heli完成签到,获得积分10
34秒前
如意枫叶发布了新的文献求助10
35秒前
都选C发布了新的文献求助10
36秒前
英俊的铭应助淡烟流水采纳,获得10
37秒前
37秒前
Miracle完成签到,获得积分10
39秒前
43秒前
wu发布了新的文献求助10
43秒前
忧心的听双完成签到,获得积分10
43秒前
Timon完成签到,获得积分10
44秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989242
求助须知:如何正确求助?哪些是违规求助? 3531393
关于积分的说明 11253753
捐赠科研通 3270010
什么是DOI,文献DOI怎么找? 1804868
邀请新用户注册赠送积分活动 882084
科研通“疑难数据库(出版商)”最低求助积分说明 809136