Stability of circulating microRNAs in serum

小RNA 早晨 生物标志物 中午 生物 餐食 实时聚合酶链反应 折叠变化 男科 内科学 医学 生理学 基因表达 遗传学 基因 物理 天文
作者
Tomáš Kupec,Andreas Bleilevens,Séverine Iborra,Laila Najjari,Julia Wittenborn,Jochen Maurer,Elmar Stickeler
出处
期刊:PLOS ONE [Public Library of Science]
卷期号:17 (8): e0268958-e0268958 被引量:11
标识
DOI:10.1371/journal.pone.0268958
摘要

There is a strong body of evidence by several translational studies which demonstrate the potential of circulating miRNAs as a potential biomarker in oncology. However, recent reports documented varying stability of these small RNA molecules in serum samples. The aim of our pilot study was to evaluate the stability of miRNAs in serum in relation to food intake and sample storage. Serum miRNA expression levels of 16 different miRNAs from 8 healthy volunteers were quantified by real-time PCR. 4 samples from each donor were analysed—2 samples (fasting, in the morning and after food intake, at noon) were analysed within 24h and 2 samples (fasting and after food intake, at noon) were stored at -80°C for 14 days and subsequently analysed. Student´s t-test was used to determine significant differences. The detectability of the distinct miRNA as a surrogate for the stability of these small RNA molecules was slightly altered by the storage conditions, but only a miRNA 22-3p, out of the analysed 16 miRNAs, shows significant lower dCq expression (3.821 vs. 4.530; p<0,01) by qPCR dependent on storage conditions (-80°C vs. 4°C). However, miRNA levels were not affected by food intake. The difference between samples taken in the morning (fasting) and at noon (after a normal meal) did not show any significant differences. MiRNAs can be considered to be a relatively stable tool in laboratory diagnostics, but clearly every new assay needs thorough evaluation. The stability of miRNAs documented here in healthy volunteers shows their potential in the search for innovative biomarkers in oncology.
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