微生物群
肠道微生物群
发病机制
生物
肠道菌群
粪便
粪便细菌疗法
计算生物学
生物信息学
免疫学
微生物学
抗生素
艰难梭菌
作者
Jin Shang,Yiding Zhang,Ruixue Guo,Wenli Liu,Jun Zhang,Yan Ge,Feng Wu,Wen Cui,Peipei Wang,Xuejun Zheng,Ting Wang,Yijun Dong,Jing Zhao,Sheng Wang,Jing Wang,Zhanzheng Zhao
标识
DOI:10.1002/advs.202201581
摘要
Abstract Membranous nephropathy (MN) is a common cause of nephrotic syndrome. The aim is to establish a non‐invasive diagnostic model of MN using differential gut microbiome analysis, and to explore the relationship between the gut microbiome and MN pathogenesis in vivo. 825 fecal samples from MN patients and healthy participants are collected from multiple medical centers across China. Key operational taxonomic units (OTUs) obtained through 16S rRNA sequencing are used to establish a diagnostic model. A rat model of MN is developed to explore the relationship between the gut microbiome and the pathogenesis of MN. The diversity and richness of the gut microbiome are significantly lower in patients with MN than in healthy individuals. The diagnostic model based on seven OTUs achieves an excellent efficiency of 98.36% in the training group and also achieves high efficiency in cross‐regional cohorts. In MN rat model, gut microbiome elimination prevents model establishment, but fecal microbiome transplantation restores the phenotype of protein urine. Gut microbiome analysis can be used as a non‐invasive tool for MN diagnosis. The onset of MN depends on the presence of naturally colonized microbiome. Early intervention in the gut microbiome may help reduce urinary protein level in MN.
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