Fresh fruits and vegetables are characterized by high water content, perishability and strong seasonality. In the process of supply chain, it is easy to lead to the decrease of freshness. Artificial intelligence (AI) technology is considered as an effective new means to assist the preservation of fruits and vegetables. It is used in the post-harvest, storage and cold chain transport stages of the fruits and vegetables supply chain. This paper reviews several advanced AI technologies and systematically introduces the application of these technologies in fruits and vegetables preservation. AI can achieve quality assessment, cold chain environment monitoring, and shelf-life prediction for fruits and vegetables, optimize supply chain management, and improve product traceability. The new fruits and vegetables preservation strategy based on AI has greatly maintained the product quality of fruits and vegetables. In addition, the current limitations and future development trends of AI technology in fruit and vegetable preservation are also discussed. This paper aims to provide guidelines for applying AI technology in fruit and vegetable preservation and provide new ideas for the future fruit and vegetable preservation process to make it more efficient and intelligent.