埃
分子内力
准确度和精密度
化学
物理
结晶学
立体化学
量子力学
作者
Steffen J. Sahl,Jessica Matthias,Kaushik Inamdar,Michael Weber,Taukeer A. Khan,Christian Brüser,Stefan Jakobs,Stefan Becker,Christian Griesinger,Johannes Broichhagen,Stefan W. Hell
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2024-10-10
卷期号:386 (6718): 180-187
标识
DOI:10.1126/science.adj7368
摘要
Optical investigations of nanometer distances between proteins, their subunits, or other biomolecules have been the exclusive prerogative of Förster resonance energy transfer (FRET) microscopy for decades. In this work, we show that MINFLUX fluorescence nanoscopy measures intramolecular distances down to 1 nanometer-and in planar projections down to 1 angstrom-directly, linearly, and with angstrom precision. Our method was validated by quantifying well-characterized 1- to 10-nanometer distances in polypeptides and proteins. Moreover, we visualized the orientations of immunoglobulin subunits, applied the method in human cells, and revealed specific configurations of a histidine kinase PAS domain dimer. Our results open the door for examining proximities and interactions by direct position measurements at the intramacromolecular scale.
科研通智能强力驱动
Strongly Powered by AbleSci AI