后肢
机器灌注
高架桥
移植
生物医学工程
生物
解剖
外科
医学
肝移植
作者
Irina Filz von Reiterdank,Paul Tawa,Yanis Berkane,Eloi de Clermont-Tonnerre,Antonia T. Dinicu,Casie A. Pendexter,Marion Goutard,Alexandre G. Lellouch,Aebele B. Mink van der Molen,J. Henk Coert,CL Cetrulo,Korkut Uygun
出处
期刊:Cryobiology
[Elsevier]
日期:2024-08-24
卷期号:116: 104950-104950
标识
DOI:10.1016/j.cryobiol.2024.104950
摘要
Ischemia is a major limiting factor in Vascularized Composite Allotransplantation (VCA) as irreversible muscular injury can occur after as early as 4-6 h of static cold storage (SCS). Organ preservation technologies have led to the development of storage protocols extending rat liver ex vivo preservation up to 4 days. Development of such a protocol for VCAs has the added challenge of inherent ice nucleating factors of the graft, therefore, this study focused on developing a robust protocol for VCA supercooling. Rodent partial hindlimbs underwent subnormothermic machine perfusion (SNMP) with several loading solutions, followed by a storage solution with cryoprotective agents (CPA) developed for VCAs. Storage occurred in suspended animation for 24h and VCAs were recovered using SNMP with modified Steen. This study shows a robust VCA supercooling preservation protocol in a rodent model. Further optimization is expected to allow for its application in a transplantation model, which would be a breakthrough in the field of VCA preservation.
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