吞吐量
病菌
生物技术
计算生物学
生化工程
计算机科学
业务
工程类
生物
微生物学
电信
无线
作者
Yundi Yin,Guiling Xiang,Yun-long Pan,Liu He,Huiyu Xu,Wenhua Wei,Li Wang,Zhong Liu
标识
DOI:10.1016/j.scitotenv.2024.174803
摘要
Effective pathogen inactivation is highly desired in public health but limited by existing methods each capable of assessing pathogen inactivation effectiveness (PIE) only in a specific condition. We therefore developed a novel method maxPIE designed to identify maximal PIEs across inactivation conditions by leveraging the power of massive array technologies. maxPIE implements a three-step algorithm to quickly identify maximal PIEs of inactivation treatments: (1) dilute pathogens into different initial titers each stored in an array well, (2) submit one sorted array to one treatment, (3) scan the treated array to find the maximum. maxPIE outperformed the conventional methods in (a) inactivating S. aureus using ultraviolet light of different wavelengths with different durations; (b) antibiotic treatment of S. aureus, E. coli, and multidrug-resistant E. coli; (c) inactivating S. aureus in plasma using ultraviolet light in different wavelengths with and without riboflavin. maxPIE was easy to understand and interpret and was robust in situations where conventional PIE methods would suffer. Hence, maxPIE can serve as an innovative and high throughput approach that can be widely used to enhance pathogen inactivation practices.
科研通智能强力驱动
Strongly Powered by AbleSci AI