MAPK/ERK通路
子宫内膜异位症
p38丝裂原活化蛋白激酶
氧化应激
细胞生物学
癌症研究
信号转导
纤维化
化学
医学
内科学
生物
作者
Xiaoqing Luo,Sixi Wen,Jing Zeng,Jing Liu,Wenting Ye,Jiangpeng Wu,Songyu Huang,Wuwei Xie,Haiping Wen,Yan Sun,Jing Cai,Delin Mo,Qian-xia Lin,Mingwei Chen,Siyu Xia,Yali Song
标识
DOI:10.1016/j.repbio.2024.100950
摘要
Epithelial-mesenchymal transition (EMT) is known to play a crucial role in the development of endometriosis (EMs). However, the exact mechanisms involved in EMT regulation in EMs are not well understood. In this study, we performed comprehensive research using clinical samples, single-cell sequencing, and in vivo/in vitro models to investigate the effects of advanced oxidation protein products (AOPPs) on EMT and the underlying mechanisms in EMs. Combining bioinformatics analysis with experimental validation, our results show that AOPPs accumulate in EMs tissues, and their levels positively correlate with the expression of EMT markers in fibrotic lesions of EMs patients. Stimulation with AOPPs leads to a concentration- and time-dependent alteration of EMT markers expression in both in vitro and in vivo models. These effects are mainly mediated by the generation of reactive oxygen species and nitrite, along with the activation of the ERK and P38 signaling pathways. In chronic administration studies using normal rats, AOPPs induce EMT and enhance collagen deposition. These findings significantly contribute to our understanding of the molecular mechanisms of EMs and provide a foundation for future research and therapeutic development in this field.
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