Cadmium isotope fractionation and gene expression evidence for tracking sources of Cd in grains during grain filling in a soil-rice system

Grain filling is the key period that causes excess cadmium (Cd) accumulation in rice grains. Nevertheless, uncertainties remain in distinguishing the multiple sources of Cd enrichment in grains. To better understand the transport and redistribution of Cd to grains upon drainage and flooding during grain filling, Cd isotope ratios and Cd-related gene expression were investigated in pot experiments. The results showed that the Cd isotopes in rice plants were much lighter than those in soil solutions (∆114/110Cdrice-soil solution = −0.36 to −0.63 ‰) but moderately heavier than those in Fe plaques (∆114/110Cdrice-Fe plaque = 0.13 to 0.24 ‰). Calculations revealed that Fe plaque might serve as the source of Cd in rice (69.2 % to 82.6 %), particularly upon flooding at the grain filling stage (82.6 %). Drainage at the grain filling stage yielded a larger extent of negative fractionation from node I to the flag leaves (∆114/110Cdflag leaves-node I = -0.82 ± 0.03 ‰), rachises (∆114/110Cdrachises-node I = -0.41 ± 0.04 ‰) and husks (∆114/110Cdrachises-node I = -0.30 ± 0.02 ‰), and significantly upregulated the OsLCT1 (phloem loading) and CAL1 (Cd-binding and xylem loading) genes in node I relative to that upon flooding. These results suggest that phloem loading of Cd into grains and transport of Cd-CAL1 complexes to flag leaves, rachises and husks were simultaneously facilitated. Upon flooding of grain filling, the positive fractionation from the leaves, rachises and husks to the grains (∆114/110Cdflag leaves/rachises/husks-node I = 0.21 to 0.29 ‰) is less pronounced than those upon drainage (∆114/110Cdflag leaves/rachises/husks-node I = 0.27 to 0.80 ‰). The CAL1 gene in flag leaves is down-regulated relative to that upon drainage. Thus, the supply of Cd from the leaves, rachises and husks to the grains is facilitated during flooding. These findings demonstrate that the excess Cd was purposefully transported to grain via xylem-to-phloem within nodes I upon the drainage during grain filling, and the expression of genes responsible for encoding ligands and transporters together with isotope fractionation could be used to tracking the source of Cd transported to rice grain.