In-situ growth of enzyme/copper phosphate hybrids on carbon cloth surface as self-powered electrochemical glucose biosensor

Enzymatic self-powered biosensors have been widely studied due to their advantages of high selectivity, simple assembly, small size, low cost and no external power supply. However, the low activity and stability of the enzyme lead to lower power density and short service life of the sensor, which seriously limits its practical application. Herein, a self-powered electrochemical biosensor (SPEB) based on in situ growth of enzyme/copper phosphate on carbon cloth surface was developed through chemical liquid coprecipitation technology. The biosensor has a higher power density of 547.97 μW·cm−3, the linear detection range of 0.1–0.5 mM, and the lowest detection limit of 2.05 μM. The unique electrochemical performance of the as-prepared biosensor is mainly due to the special three-dimensional (3D) flower structure of the enzyme/copper phosphate organic/inorganic hybrids, which has a unique flower-like structure and high specific surface area, allowing more exposure to the active site of the enzyme. Moreover, the enzyme has a synergistic effect with Cu3(PO4)2, which not only ensures the specificity of the enzyme, but also improves the catalytic activity and stability of the enzyme, improving the performance of SPEB. The SPEB is expected to be applied to glucose detection.