核酸酶
DNA
生物传感器
滚动圆复制
电化学发光
核酸内切酶
二茂铁
生物物理学
A-DNA
检出限
复式(建筑)
化学
组合化学
分子生物学
纳米技术
材料科学
电极
生物化学
生物
DNA聚合酶
色谱法
电化学
物理化学
作者
Mingyue Wang,Wen-Jie Jing,Lijuan Wang,Liping Jia,Rong-Na Ma,Wei Zhang,Lei Shang,Xiaojian Li,Qingwang Xue,Huaisheng Wang
标识
DOI:10.1016/j.bios.2023.115116
摘要
DNA nanomachines have shown potential application in the construction of various biosensors. Here, an electrochemiluminescence biosensor for the sensitive detection of miRNA-21 were reported based on three-dimensional (3D) DNA nanomachine and duplex-specific nuclease (DSN)-mediated target recycle amplification strategy. First, the bipedal DNA walkers were obtained by DSN-mediated digestion reaction initiated by target miRNA-21.3D DNA tracks were prepared by modifying Fe3O4 magnetic beads (MBs) with ferrocene-labeled DNA (Fc-DNA). The produced DNA walkers autonomously moved along 3D DNA tracks powered by nicking endonuclease. During the movement, ferrocene-labeled DNA was cleaved, resulting in large amounts of Fc-labeled DNA fragments away from the MBs surface. Finally, the liberated Fc-labeled DNA fragments were dropped on the C-g-C3N4 modified electrode surface, leading to the quenching of C-g-C3N4 electrochemiluminescence (ECL). Benefiting from the dual amplification strategy of 3D DNA nanomachine and DSN-mediated target recycling, the developed ECL biosensor exhibited an excellent performance for miRNA-21 detection with a wide linear range of 10 fM to 10 nM and a low detection limit of 1.0 fM. This work offers a new thought for the application of DNA walkers in the construction of various biosensors.
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