多巴胺
抗坏血酸
胚胎干细胞
细胞分化
干细胞
石墨烯
神经干细胞
化学
纳米技术
神经元
细胞生物学
材料科学
生物
生物化学
神经科学
基因
食品科学
作者
Dehua Yang,Ting Li,Minghan Xu,Feng Gao,Juan Yang,Zhi Yang,Weidong Le
出处
期刊:Nanomedicine
日期:2014-02-24
卷期号:9 (16): 2445-2455
被引量:125
摘要
Aim: Nanoparticles are easier to pass through cell membranes, and they are considered to be the ideal biocompatible and mechanically stable platforms for supporting stem cell growth and differentiation. The aim of this study is to determine the effects of carbon nanotubes (CNTs), graphene oxide (GO) and graphene (GR) on the dopamine neural differentiation of mouse embryonic stem cells (ESCs). Materials & methods: GO was prepared according to a modified Hummers method. GR was synthesized by reduction of GO via L-ascorbic acid as a reductant in an aqueous solution at room temperature. CNTs were fabricated by chemical vapor deposition method. ESCs were differentiated by a stromal cell-derived inducing activity (SDIA) method after 10 days coculture with PA6 cells. The dopamine neural differentiation of the ESCs-GFP was examined by immunocytochemistry and real-time PCR. Results: We found that only GO could effectively promote dopamine neuron differentiation after induction of SDIA and further enhance dopamine neuron-related gene expression compared with cells treated with no nanoparticle control, and the other two nanoparticles (CNTs and GR). Conclusion: These findings suggest that GO is a promising nanomaterial-based technical platform to effectively enhance dopamine neural differentiation of ESCs, which can be potentially applied for cell transplantation therapy. Original submitted 22 April 2013; Revised submitted 13 October 2013
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