酿酒酵母
磷酸三酯异构酶
丙二醇
达普
质粒
甲基乙二醛
磷酸二羟丙酮
生物
生物化学
1,3-丙二醇
甘油
代谢工程
基因
化学
微生物学
酶
有机化学
作者
Joon Young Jung,Eun Sil Choi,Min‐Kyu Oh
出处
期刊:Journal of Microbiology and Biotechnology
[Journal of Microbiology and Biotechnology]
日期:2008-11-28
卷期号:: 1797-1802
被引量:36
摘要
Saccharomyces cerevisiae was metabolically engineered to improve 1,2-propanediol production. Deletion of the tpi1 (triosephosphate isomerase) gene in S. cerevisiae increased the carbon flux to DHAP (dihydroxylacetone phosphate) in glycolysis, resulting in increased glycerol production. Then, the mgs and gldA genes, the products of which convert DHAP to 1,2-propanediol, were introduced to the tpi1-deficient strain using a multicopy plasmid. As expected, the intracellular level of methylglyoxal was increased by introduction of the mgs gene in S. cerevisiae and that of 1,2-propanediol by introduction of both the mgs and gldA genes. As a result, 1.11 g/l of 1,2-propanediol was achieved in flask culture.
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