多利醇
内质网
生物化学
生物合成
酵母
突变体
生物
免疫沉淀
糖基化
细胞生物学
酶
基因
作者
Megan I. Brasher,Liliana Surmacz,Bryan J. Leong,Jocelyn Pitcher,Ewa Świeżewska,Eran Pichersky,Tariq A. Akhtar
出处
期刊:Plant Journal
[Wiley]
日期:2015-04-21
卷期号:82 (6): 903-914
被引量:58
摘要
Summary Dolichol plays an indispensable role in the N ‐glycosylation of eukaryotic proteins. As proteins enter the secretory pathway they are decorated by a ‘glycan’, which is preassembled onto a membrane‐anchored dolichol molecule embedded within the endoplasmic reticulum ( ER ). Genetic and biochemical evidence in yeast and animals indicate that a cis ‐prenyltransferase ( CPT ) is required for dolichol synthesis, but also point to other factor(s) that could be involved. In this study, RNA i‐mediated suppression of one member of the tomato CPT family (Sl CPT 3) resulted in a ~60% decrease in dolichol content. We further show that the involvement of Sl CPT 3 in dolichol biosynthesis requires the participation of a distantly related partner protein, designated as CPT ‐binding protein (Sl CPTBP ), which is a close homolog of the human Nogo‐B receptor. Yeast two‐hybrid and co‐immunoprecipitation assays demonstrate that Sl CPT 3 and its partner protein interact in vivo and that both Sl CPT 3 and Sl CPTBP are required to complement the growth defects and dolichol deficiency of the yeast dolichol mutant, rer2∆ . Co‐expression of Sl CPT 3 and Sl CPTBP in yeast and in E . coli confirmed that dolichol synthase activity strictly requires both proteins. Finally, organelle isolation and in vivo localization of fluorescent protein fusions showed that both Sl CPT 3 and Sl CPTBP localize to the ER , the site of dolichol accumulation and synthesis in eukaryotes.
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