生物
温度梯度凝胶电泳
反硝化细菌
放大器
聚合酶链反应
凝胶电泳
基因
分子生物学
遗传学
16S核糖体RNA
反硝化
量子力学
物理
氮气
作者
Ingela Noredal Throbäck,Karin Enwall,Åsa Jarvis,Sara Hallin
标识
DOI:10.1016/j.femsec.2004.04.011
摘要
We re-evaluated PCR primers targeting nirS, nirK and nosZ genes for denaturing gradient gel electrophoresis as a tool to survey denitrifying community composition in environmental samples. New primers for both nirS and nosZ were combined with existing primers, while for nirK the previously published F1aCu:R3Cu set was chosen for denaturing electrophoresis. All three sets yielded amplicons smaller than 500 bp and amplified the correct fragment in all environmental samples. The denaturing gradient gel electrophoresis worked satisfactorily for nirK and nosZ, but not for nirS. This was probably due to the multiple melting domains in this particular nirS fragment. From the excised and sequenced bands, only sequences related to the target genes were detected and tree analysis showed that the selected primers acted as broad range primers for each of the three genes. By use of the new nirS primers it was demonstrated that agricultural soil harbours a substantial diversity of nirS denitrifiers.
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