NAD+激酶
生物化学
转移RNA
烟酰胺腺嘌呤二核苷酸
酿酒酵母
核糖
磷酸转移酶
化学
脱磷
核糖核酸
酵母
磷酸酶
酶
基因
作者
Gloria M. Culver,Stephen M. McCraith,Martin Zillmann,Ryszard Kierzek,Neil R. Michaud,Richard D. LaReau,Douglas H. Turner,Eric M. Phizicky
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:1993-07-09
卷期号:261 (5118): 206-208
被引量:96
标识
DOI:10.1126/science.8392224
摘要
Transfer RNA (tRNA) splicing is essential in Saccharomyces cerevisiae as well as in humans, and many of its features are the same in both. In yeast, the final step of this process is removal of the 2′ phosphate generated at the splice junction during ligation. A nicotinamide adenine dinucleotide (NAD)-dependent phosphotransferase catalyzes removal of the 2′ phosphate and produces a small molecule. It is shown here that this small molecule is an NAD derivative: adenosine diphosphate (ADP)-ribose 1"-2" cyclic phosphate. Evidence is also presented that this molecule is produced in Xenopus laevis oocytes as a result of dephosphorylation of ligated tRNA.
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