In vitro antioxidant activities and HPTLC analysis of ethanolic extract of Cayratia trifolia (L.)

To assess the preliminary phytochemical constituents, free radical scavenging activities and HPTLC analysis of the whole plant of the ethanolic extract of Cayratia trifolia. 2, 2- diphenyl-1-picryl-hydrazyl, reducing power assay, superoxide radical scavenging, hydroxyl scavenging and nitric oxide scavenging assays were used in this study. The HPTLC analysis was also performed to confirm the presence of alkaloids and flavonoids in the ethanolic extract of Cayratia trifolia. The preliminary phytochemical screening has shown the presence of alkaloid, flavonoids, tannins, saponins and phenolic compounds. The ethanolic extract of Cayratia trifolia possesses the free radical scavenging activities. The HPTLC analysis assessed that, the ethanolic extract of Cayratia trifolia has five alkaloid compounds and one flavonoid compounds. It is concluded that, the Cayratia trifolia contains natural source of antioxidants and the ethanolic extract of this plant possess a good free radical scavenging activity. In future, it can be used as a bioactive source of natural antioxidants and are potential natural resources for pharmacology of functional foods.