量子点
结合
荧光
纳米晶
共轭体系
化学
直接荧光抗体
抗体
罗丹明
免疫荧光
分子生物学
材料科学
生物
物理
纳米技术
光学
免疫学
数学
聚合物
有机化学
数学分析
作者
Xiaoling Tang,Jun He,James Partin,Abbas Vafai
标识
DOI:10.1080/10739149.2010.488620
摘要
A comparative analysis was performed to determine the sensitivity and efficiency of three fluorescent labeling techniques, including direct fluorescent-antibody staining (FA), Zenon labeling, and quantum dot (QD) nanocrystal technology. Two varicella-zoster virus immunoglobin (Ig) G forms, mAb 4F9 and mAb g62, were selected for these studies. The results indicated that: (1) All three methods demonstrated similar brightness and photostability; (2) the time required to conjugate the antibody varied, with Zenon labeling being the quickest; and (3) the stability of each conjugated complex was different, with FITC/rhodamine-conjugated antibody being the most stable.
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