化学
半胱氨酸
醛
赫普斯
荧光
荧光素
谷胱甘肽
同型半胱氨酸
转身(生物化学)
检出限
分子内力
光化学
生物化学
立体化学
色谱法
催化作用
酶
物理
量子力学
摘要
We developed a fluorescein aldehyde probe with disulfide functionality for the fluorescence detection of biologically important thiols. The probe displayed highly selective responses to cysteine (Cys) and homocysteine (Hcy) over glutathione (GSH) due to the rapid ring formation reaction of Cys and Hcy with the aldehyde group of the probe and the concomitant cleavage of the disulfide group followed by subsequent intramolecular cyclization. The fluorescent probe also exhibited a highly sensitive fluorescence turn-on response to Hcy with a detection limit of 2.4 μM Hcy in HEPES buffer.
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