昆虫
Cas9
基因敲除
清脆的
基因
基因组编辑
生物
计算生物学
化学
细胞生物学
生物技术
遗传学
植物
作者
Jingxiang Chen,Yu‐Tao Hu,Chuan-Chuan Zhang,Min-Shi Luo,Wenqing Zhang
标识
DOI:10.1016/j.ijbiomac.2024.135413
摘要
Knockouts mediated by CRISPR/Cas9 technology are widely used to study insect gene functions, but the efficiency in Hemiptera is low. New strategies are urgently needed to improve gene knockout efficiency. This study initially explored the impact of modifying the fundamental backbone structure of single guide RNA (sgRNA) on knockout efficiency. The results indicated that both in vitro and in vivo transcription of sgRNA structures (Loop
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