岩藻糖基化
化学
岩藻糖基转移酶
聚糖
岩藻糖
免疫沉淀
生物化学
酶
糖基转移酶
细胞
糖蛋白
基因
作者
Qiang Zhu,Jean-Luc Chaubard,Didi Geng,Jiechen Shen,Lan Ban,Sheldon T. Cheung,Fangyu Wei,Yating Liu,Haofan Sun,Angie Calderon,Wenbo Dong,Weijie Qin,Tiehai Li,Liuqing Wen,Peng George Wang,Shisheng Sun,Wen Yi,Linda C. Hsieh‐Wilson
摘要
Core fucosylation, the attachment of an α-1,6-linked-fucose to the N-glycan core pentasaccharide, is an abundant protein modification that plays critical roles in various biological processes such as cell signaling, B cell development, antibody-dependent cellular cytotoxicity, and oncogenesis. However, the tools currently used to detect core fucosylation suffer from poor specificity, exhibiting cross-reactivity against all types of fucosylation. Herein we report the development of a new chemoenzymatic strategy for the rapid and selective detection of core fucosylated glycans. This approach employs a galactosyltransferase enzyme identified from
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