抗抑郁药
代谢组学
汤剂
生物
药理学
脂质代谢
肠道菌群
拟杆菌
医学
生物信息学
神经科学
海马体
生物化学
16S核糖体RNA
植物
基因
作者
Wanru Dong,Shenqiang Hu,Jin Yü,Ying Liu,ShuTing Zeng,Xiaodong Liu,Yongchun Deng,Li Wang,Jian Yin,Botao Xing,Zunpeng Shu
出处
期刊:Combinatorial Chemistry & High Throughput Screening
[Bentham Science]
日期:2024-10-03
卷期号:28
标识
DOI:10.2174/0113862073312654240918072945
摘要
Background: The incidence of depression is increasing year by year, and Zhizichi Decoction(ZZCD)has shown significant efficiency in the clinical treatment of mild depression, but its mechanism of action is still unclear. In this research, network pharmacology and metagenomics combined and metabolomics were used as research methods to explain the scientific connotation of the antidepressant effect of ZZCD from the aspects of the overall effect of organisms and microbial structure and function. Methods: The rat model of depression was established by chronic unpredictable mild stress (CUMS), and the improvement of depressive symptoms was evaluated by behavioral and histopathological methods. Network pharmacology predicted possible targets and important pathways of ZZCD. Metabolomics revealed its possible related biological pathways. Metagenomics showed the disturbance of ZZCD on intestinal microbial diversity structure and associated biological functions in depressed rats. Results: ZZCD can improve the behavioral performance of CUMS rats, and can significantly regulate the content of 5-HT, NE and other neurotransmitters in serum and brain tissue, and improve the damaged state of neurons in the hippocampus. Network pharmacology predicts that it mainly acts on biological processes such as inflammatory response and oxidative stress response. Metabolomics found that it affected metabolic pathways such as amino acid metabolism and lipid metabolism. The results of metagenomics showed that it significantly regulated the abundance of Firmicutes and Bacteroidetes. The above results predicted that it may affect signaling pathways such as the nervous system, inflammatory diseases and cell processing. Conclusion: ZZCD may play an antidepressant role by regulating intestinal probiotics, energy metabolism, and inflammation reduction. This provides a scientific basis for the clinical application of ZZCD in traditional Chinese medicine and also makes it an optional alternative for the treatment of depression.
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