深共晶溶剂
多酚氧化酶
化学
溶剂
多酚
山茶
色谱法
儿茶酚氧化酶
响应面法
萃取(化学)
共晶体系
植物
酶
有机化学
生物
抗氧化剂
合金
过氧化物酶
作者
Yuqin Xu,Bin Zeng,Shuangling Xiao,Sheng Wang,Yang Liu,Shengchang Chen,Jie Teng
标识
DOI:10.1016/j.fochx.2024.101720
摘要
In this study, tea polyphenol oxidase (PPO) was purified via three-phase partitioning (TPP) using a deep eutectic solvent (DES) instead of t-butanol. First, the properties of 13 types of synthesized DESs were characterized, and DES-7 (thymol/dodecanoic acid) was selected as the best alternative solvent. The process parameters were optimized using response surface methodology. The experimental results revealed that when the (NH4)2SO4 concentration, DES to crude extract ratio, extraction time, and pH were 41%, 0.5:1, 75 min, and 5.6, respectively, the recovery and purification fold of tea PPO were 78.44% and 8.26, respectively. SDS-PAGE and native-PAGE were used to analyze the PPO before and after purification of the TTP system, and the molecular weight and purification effect of PPO were detected. Moreover, the DES could be recovered and recycled. The results indicate an environmentally friendly and stable DES, and provide a reference for the large-scale application of TPP to extract PPO.
科研通智能强力驱动
Strongly Powered by AbleSci AI