鼻咽癌
转录组
免疫疗法
癌症研究
生物
计算生物学
医学
免疫学
基因
放射治疗
免疫系统
内科学
遗传学
基因表达
作者
Yang Liu,Shuangyan Ye,Shuai He,Dongmei Chi,Xiuzhi Wang,Yue‐Feng Wen,Dong Ma,Run‐Cong Nie,Xiang Pu,Yundong Zhou,Zhao-Hui Ruan,Roujun Peng,Chun-Ling Luo,Panpan Wei,Guo‐Wang Lin,Jian Zheng,Qian Cui,Muyan Cai,Jing‐Ping Yun,Jing Dong,Hai‐Qiang Mai,Xiaojun Xia,Jin‐Xin Bei
标识
DOI:10.1038/s41467-024-52153-4
摘要
Abstract Tertiary lymphoid structures are immune cell aggregates linked with cancer outcomes, but their interactions with tumour cell aggregates are unclear. Using nasopharyngeal carcinoma as a model, here we analyse single-cell transcriptomes of 343,829 cells from 77 biopsy and blood samples and spatially-resolved transcriptomes of 31,316 spots from 15 tumours to decipher their components and interactions with tumour cell aggregates. We identify essential cell populations in tertiary lymphoid structure, including CXCL13 + cancer-associated fibroblasts, stem-like CXCL13 + CD8 + T cells, and B and T follicular helper cells. Our study shows that germinal centre reaction matures plasma cells. These plasma cells intersperse with tumour cell aggregates, promoting apoptosis of EBV-related malignant cells and enhancing immunotherapy response. CXCL13 + cancer-associated fibroblasts promote B cell adhesion and antibody production, activating CXCL13 + CD8 + T cells that become exhausted in tumour cell aggregates. Tertiary lymphoid structure-related cell signatures correlate with prognosis and PD-1 blockade response, offering insights for therapeutic strategies in cancers.
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