检出限
微球
荧光
色谱法
重复性
碳二亚胺
化学
材料科学
化学工程
生物化学
物理
量子力学
工程类
作者
Zongyou Chen,Yanyan Tang,Ping Guo,Wei Zhang,Juan Peng,Yonghua Xiong,Bingfeng Ma,Weihua Lai
出处
期刊:Food Chemistry
[Elsevier]
日期:2024-04-04
卷期号:450: 139260-139260
被引量:8
标识
DOI:10.1016/j.foodchem.2024.139260
摘要
High fluorescence intensity microspheres such as aggregation-induced emission fluorescence microspheres (AIEFM) have improved the sensitivity of lateral flow immunoassay (LFIA). The preparation of immune probes in LFIA usually adopts the chemical coupling strategy with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide for antibody coupling, which has the problems of low coupling efficiency, tedious coupling process, and poor repeatability. A biocompatible metal-phenolic network (MPN), which contains large amounts of phenols and galloyl groups, could easily, quickly, and stably couple with antibodies. Herein, we proposed a strategy based on MPN modification on ultrabright AIEFM surface as a novel label for the rapid detection of carbendazim. The limit of detection of AIEFM@MPN-LFIA was 0.019 ng/mL, which was 4.9 times lower than that of AIEFM-LFIA. In spiked samples, the average recoveries of AIEFM@MPN-LFIA ranged from 80% to 118% (coefficient of variation <13.45%). Therefore, AIEFM@MPN was a promising signal label that could improve the detection performance of LFIA.
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