色谱法
化学
亲水作用色谱法
分馏
质谱法
大小排阻色谱法
反相色谱法
固定相
离子色谱法
电喷雾
高效液相色谱法
样品制备
色谱柱
生物化学
酶
作者
Luiz Fernando Ribeiro,Jiřı́ Urban
标识
DOI:10.1002/jssc.202300174
摘要
In this article, we review stationary phase‐assisted sample prefractionation in proteomic analysis in 2019–2022 period. Applications are grouped according to the mode of retention used in the prefractionation step prior to the final analysis by low‐pH reversed‐phase liquid chromatography‐tandem mass spectrometry. Online or offline instrumental configurations are also commented on, with a highlight on novel online platforms. From the articles reviewed in this period, the order of popularity of chromatographic modes for sample fractionation is affinity chromatography > size exclusion chromatography > hydrophilic interaction chromatography > high‐pH reversed‐phase liquid chromatography > ion exchange chromatography.
科研通智能强力驱动
Strongly Powered by AbleSci AI