Recent progress of near-infrared fluorescent probes in the determination of reactive oxygen species for disease diagnosis

Reactive oxygen species (ROS), a chemically defined group of reactive molecules derived from molecular oxygen, are involved in a variety of physiological and pathological processes, including immune defense, cellular metabolism, and other physiological processes. To access their detailed function in these processes, it is critical to establish rapid, accurate and in situ assays for these species in vivo. Among the potential assays, fluorescent probes are considered as the most promising candidate to monitor the biological ROS in vivo with great spatial and temporal resolution and are extensively used as an excellent tool in modern redox biology discovery. Recently, abundant fluorescent probes have been successively developed for in vitro or intracellular detection of ROS, but most of them could not be used for in vivo imaging due to their intrinsic shortcomings such as short emission wavelengths, phototoxicity and poor tissue penetration. Recent development of fluorescent ROS probes with near-infrared emission aim to address these concerns to develop practical assays. Herein, we review recent developments of ROS-sensitive near-infrared fluorescent probes, with an emphasis on the design, synthesis, characteristics of fluorescent probes, as well as their applications. We hope this review will aid the development of a new generation of efficient, sensitive and biocompatible fluorescent probes for in vivo ROS detection.