癌症干细胞
转移
波形蛋白
CD24型
上皮-间质转换
癌症研究
癌细胞
癌症
病理
CD44细胞
干细胞标记物
传播
间充质干细胞
干细胞
生物
细胞
医学
免疫组织化学
内科学
计算机科学
细胞生物学
电信
遗传学
作者
Gehad Youssef,Luke Gammon,Leah Ambler,Sophia Lunetto,Alice Scemama,Hannah Cottom,Kim Piper,Ian C. Mackenzie,Michael P. Philpott,Adrian Biddle
出处
期刊:eLife
[eLife Sciences Publications, Ltd.]
日期:2023-11-17
卷期号:12
摘要
Cancer stem cells (CSCs) undergo epithelial-mesenchymal transition (EMT) to drive metastatic dissemination in experimental cancer models. However, tumour cells undergoing EMT have not been observed disseminating into the tissue surrounding human tumour specimens, leaving the relevance to human cancer uncertain. We have previously identified both EpCAM and CD24 as CSC markers that, alongside the mesenchymal marker Vimentin, identify EMT CSCs in human oral cancer cell lines. This afforded the opportunity to investigate whether the combination of these three markers can identify disseminating EMT CSCs in actual human tumours. Examining disseminating tumour cells in over 12,000 imaging fields from 74 human oral tumours, we see a significant enrichment of EpCAM, CD24 and Vimentin co-stained cells disseminating beyond the tumour body in metastatic specimens. Through training an artificial neural network, these predict metastasis with high accuracy (cross-validated accuracy of 87-89%). In this study, we have observed single disseminating EMT CSCs in human oral cancer specimens, and these are highly predictive of metastatic disease.
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