Rapid and Sensitive Quantification of Bacterial Viability Using Ratiometric Fluorescence Sensing

化学 荧光 色谱法 光学 物理
作者
Shengbin He,Yajing Chen,Jingtong Wang,Jian Sun,Xinyi Zhang,Quanzhi Chen
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:96 (27): 11018-11025 被引量:7
标识
DOI:10.1021/acs.analchem.4c01737
摘要

Bacterial viability assessment plays an important role in food-borne pathogen detection and antimicrobial drug development. Here, we first used GelRed as a DNA-binding stain for a bacterial viability assessment. It was found that live bacteria were able to exclude GelRed, which however could easily penetrate dead ones and be absorbed nonspecifically on the bacterial periplasm. Cations were used to reduce the nonspecific adsorption and greatly increase the red fluorescence ratio of dead to live bacteria. Combined with SYTO 9 (a membrane-permeable dye) for double-staining, a ratiometric fluorescent method was established. Using Escherichia coli O157:H7 as a bacteria model, the ratiometric fluorescent method can probe dead bacteria as low as 0.1%. A linear correlation between the ratiometric fluorescence and the theoretical ratio of dead bacteria was acquired, with a correlation coefficient R2 of 0.97. Advantages in sensitivity, accuracy, and safety of the GelRed/SYTO9-based ratiometric fluorescent method against traditional methods were demonstrated. The established method was successfully applied to the assessment of germicidal efficacy of different heat treatments. It was found that even 50 °C treatment could lead to the death of minor bacteria. The as-developed method has many potential applications in microbial researches, and we believe it could be expanded to the viability assessment of mammalian cells.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
开朗的汉堡完成签到,获得积分10
1秒前
wxs完成签到,获得积分10
2秒前
浮华乱世完成签到 ,获得积分10
3秒前
但大图完成签到 ,获得积分0
4秒前
dujinjun完成签到,获得积分10
7秒前
lysixsixsix完成签到,获得积分10
10秒前
渴望者完成签到,获得积分10
11秒前
大橙子发布了新的文献求助10
11秒前
ZQ完成签到,获得积分10
18秒前
小包子完成签到,获得积分10
19秒前
liyan完成签到 ,获得积分10
20秒前
21秒前
嗯啊完成签到,获得积分10
23秒前
酷波er应助immm采纳,获得10
24秒前
优雅含莲完成签到 ,获得积分10
24秒前
呜啦啦完成签到,获得积分10
25秒前
25秒前
lulu8809完成签到,获得积分10
28秒前
28秒前
二十五完成签到,获得积分10
29秒前
romeo完成签到,获得积分10
30秒前
kaka完成签到 ,获得积分10
30秒前
Akim应助xialuoke采纳,获得10
30秒前
昏睡的蟠桃应助guoxingliu采纳,获得200
31秒前
慕容松完成签到,获得积分10
32秒前
romeo发布了新的文献求助10
32秒前
ss_hHe完成签到,获得积分10
33秒前
33秒前
34秒前
zjcomposite完成签到,获得积分10
34秒前
nn发布了新的文献求助10
34秒前
css完成签到,获得积分10
34秒前
大橙子发布了新的文献求助10
35秒前
1111完成签到,获得积分10
35秒前
敏er好学完成签到,获得积分10
36秒前
细腻的谷秋完成签到 ,获得积分10
36秒前
独特的易形完成签到,获得积分10
37秒前
yangyangyang完成签到,获得积分0
40秒前
yirenli完成签到,获得积分10
41秒前
叶子完成签到 ,获得积分10
41秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038201
求助须知:如何正确求助?哪些是违规求助? 3575940
关于积分的说明 11373987
捐赠科研通 3305747
什么是DOI,文献DOI怎么找? 1819274
邀请新用户注册赠送积分活动 892662
科研通“疑难数据库(出版商)”最低求助积分说明 815022