A Thiol-Anchored Solvatochromic and Fluorogenic Molecular Rotor for Covalent Protein Labeling in SDS-PAGE and Mitochondria Specific Fluorescence Imaging

Fluorescent labeling is a widely used method for protein detection and fluorescence imaging. A solvatochromic and fluorogenic molecular rotor DASPBCl was developed for covalent protein labeling in solution and SDS-PAGE, and also for stable mitochondria labeling and fluorescence imaging. The dye DASPBCl consisted of a 4-(