Ame‐miR‐1‐3p of bee venom reduced cell viability through the AZIN1/OAZ1‐ODC1‐polyamines pathway and enhanced the defense ability of honeybee (Apis mellifera L.)

生物 小RNA 转录组 活力测定 基因 细胞生物学 基因表达 遗传学 细胞
作者
Haifeng Liu,Tian Xue,Jie Wen,Jie Liu,Yunfei Huo,Kangqi Yuan,Jiazhong Guo,Xun Wang,Mingxian Yang,Anan Jiang,Quanquan Cao,Jun Jiang
出处
期刊:Insect Molecular Biology [Wiley]
卷期号:33 (4): 312-322
标识
DOI:10.1111/imb.12899
摘要

Abstract Bee venom serves as an essential defensive weapon for bees and also finds application as a medicinal drug. MicroRNAs (miRNAs) serve as critical regulators and have been demonstrated to perform a variety of biological functions. However, the presence of miRNAs in bee venom needs to be confirmed. Therefore, we conducted small RNA sequencing and identified 158 known miRNAs, 15 conserved miRNAs and 4 novel miRNAs. It is noteworthy that ame‐miR‐1‐3p, the most abundant among them, accounted for over a quarter of all miRNA reads. To validate the function of ame‐miR‐1‐3p, we screened 28 candidate target genes using transcriptome sequencing and three target gene prediction software (miRanda, PITA and TargetScan) for ame‐miR‐1‐3p. Subsequently, we employed real‐time quantitative reverse transcription PCR (qRT‐PCR), Western blot and other technologies to confirm that ame‐miR‐1‐3p inhibits the relative expression of antizyme inhibitor 1 (AZIN1) by targeting the 3′ untranslated region (UTR) of AZIN1. This, in turn, caused ODC antizyme 1 (OAZ1) to bind to ornithine decarboxylase 1 (ODC1) and mark ODC1 for proteolytic destruction. The reduction in functional ODC1 ultimately resulted in a decrease in polyamine biosynthesis. Furthermore, we determined that ame‐miR‐1‐3p accelerates cell death through the AZIN1/OAZ1‐ODC1‐polyamines pathway. Our studies demonstrate that ame‐miR‐1‐3p diminishes cell viability and it may collaborate with sPLA2 to enhance the defence capabilities of honeybees ( Apis mellifera L.). Collectively, these data further elucidate the defence mechanism of bee venom and expand the potential applications of bee venom in medical treatment.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
金色热浪完成签到,获得积分10
1秒前
闫伯涵完成签到,获得积分10
2秒前
CodeCraft应助美丽访云采纳,获得20
2秒前
2秒前
Gates发布了新的文献求助10
2秒前
3秒前
大模型应助qiaoqiao采纳,获得10
3秒前
4秒前
4秒前
Jar发布了新的文献求助10
4秒前
小巧孤菱完成签到,获得积分10
4秒前
bkagyin应助杨天水采纳,获得10
5秒前
赘婿应助闫伯涵采纳,获得10
6秒前
6秒前
yanjiusheng完成签到,获得积分10
7秒前
斯文的白玉完成签到 ,获得积分10
7秒前
郑qqqq发布了新的文献求助10
7秒前
7秒前
mlml发布了新的文献求助10
7秒前
可意发布了新的文献求助20
8秒前
8秒前
simon完成签到,获得积分10
8秒前
9秒前
MG_aichy发布了新的文献求助10
9秒前
鱼咬羊发布了新的文献求助10
9秒前
启航完成签到,获得积分10
9秒前
小强呀完成签到,获得积分20
9秒前
任性的小懒猪完成签到 ,获得积分10
9秒前
顺利白桃完成签到,获得积分0
10秒前
SEULE完成签到,获得积分10
10秒前
三里墩头应助慈祥的天曼采纳,获得10
10秒前
10秒前
10秒前
Cherry完成签到,获得积分10
11秒前
crazygg发布了新的文献求助10
11秒前
11秒前
11秒前
11秒前
烟花应助zhangwei采纳,获得10
12秒前
小强呀发布了新的文献求助10
12秒前
高分求助中
Production Logging: Theoretical and Interpretive Elements 2700
Mechanistic Modeling of Gas-Liquid Two-Phase Flow in Pipes 2500
Kelsen’s Legacy: Legal Normativity, International Law and Democracy 1000
Handbook on Inequality and Social Capital 800
Conference Record, IAS Annual Meeting 1977 610
Interest Rate Modeling. Volume 3: Products and Risk Management 600
Interest Rate Modeling. Volume 2: Term Structure Models 600
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3546689
求助须知:如何正确求助?哪些是违规求助? 3123769
关于积分的说明 9356697
捐赠科研通 2822394
什么是DOI,文献DOI怎么找? 1551413
邀请新用户注册赠送积分活动 723398
科研通“疑难数据库(出版商)”最低求助积分说明 713736