水解
生物量(生态学)
硫酸
水解物
糖
原材料
响应面法
生物能源
生物燃料
产量(工程)
发酵
制浆造纸工业
还原糖
食品科学
酸水解
木质素
生物
植物
生物化学
化学
色谱法
生物技术
材料科学
农学
有机化学
生态学
工程类
冶金
作者
Joana Oliveira,Sara Pardilhó,Joana Maia Dias,José C.M. Pires
出处
期刊:Biology
[Multidisciplinary Digital Publishing Institute]
日期:2023-06-29
卷期号:12 (7): 935-935
标识
DOI:10.3390/biology12070935
摘要
Microalgae are a promising feedstock for bioethanol production, essentially due to their high growth rates and absence of lignin. Hydrolysis—where the monosaccharides are released for further fermentation—is considered a critical step, and its optimization is advised for each raw material. The present study focuses on the thermal acid hydrolysis (with sulfuric acid) of Aurantiochytrium sp. through a response surface methodology (RSM), studying the effect of acid concentration, hydrolysis time and biomass/acid ratio on both sugar concentration of the hydrolysate and biomass conversion yield. Preliminary studies allowed to establish the range of the variables to be optimized. The obtained models predicted a maximum sugar concentration (18.05 g/L; R2 = 0.990) after 90 min of hydrolysis, using 15% (w/v) biomass/acid ratio and sulfuric acid at 3.5% (v/v), whereas the maximum conversion yield (12.86 g/100 g; R2 = 0.876) was obtained using 9.3% (w/v) biomass/acid ratio, maintaining the other parameters. Model outputs indicate that the biomass/acid ratio and time are the most influential parameters on the sugar concentration and yield models, respectively. The study allowed to obtain a predictive model that is very well adjusted to the experimental data to find the best saccharification conditions for the Aurantiochytrium sp. microalgae.
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